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Diabetes and Vascular Disease Research
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The application of proteomics to diabetes

Eleanor M Scott

Academic Unit of Molecular Vascular Medicine, The LIGHT Laboratories, Clarendon Way, University of Leeds, Leeds, LS2 9JT, UK

Angela M Carter

Academic Unit of Molecular Vascular Medicine, The LIGHT Laboratories, Clarendon Way, University of Leeds, Leeds, LS2 9JT, UK

John BC Findlay

Department of Biochemistry, Leeds University, Leeds, UK

Proteomics is the investigation of all the proteins and their various modifications making up a system, be that a cell, tissue or organism. The techniques involved in proteomics allow the global screening of complex samples of proteins and provide qualitative and quantitative evidence of altered protein expression. This lends itself to the investigation of the molecular mechanisms underpinning disease processes and the effects of treatment. This review describes the main techniques of proteomics and how they have begun to be applied to diabetes research.

Key Words: proteomics • diabetes • two-dimensional gel electrophoresis (2DE) • mass spectrometry • MALDI • ESI • SELDI

Diabetes and Vascular Disease Research, Vol. 2, No. 2, 54-60 (2005)
DOI: 10.3132/dvdr.2005.009
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