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Diabetes and Vascular Disease Research
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Human islet isolation: semi-automated and manual methods

Michelle Paget

The Islet Research Laboratory, Worcestershire Clinical Research Unit, Worcestershire Acute Hospitals NHS Trust, Newtown Road, Worcester, WR5 1HN, UK.

Hilary Murray

The Islet Research Laboratory, Worcestershire Clinical Research Unit, Worcestershire Acute Hospitals NHS Trust, Newtown Road, Worcester, WR5 1HN, UK.

Clifford J Bailey

Diabetes Research, Life and Health Sciences, Aston University, Birmingham, B4 7ET, UK.

Richard Downing

The Islet Research Laboratory, Worcestershire Clinical Research Unit, Worcestershire Acute Hospitals NHS Trust, Newtown Road, Worcester, WR5 1HN, UK.

Large yields of high-viability human islets are necessary to service the expanding programmes of islet transplantation worldwide; similarly, there is an increasing demand from diabetes researchers for a reliable and cost-effective supply of human islets. The two main isolation methods are ‘semi-automated’ and ‘manual’. Both methods rely on prompt and careful removal and transfer of the donor pancreas to allow isolation to commence, preferably within eight hours. Each method involves exocrine digestion with high-activity collagenase (Liberase).

The semi-automated method is standardised, generally provides higher islet yields and is used for clinical transplant purposes, although it is not suitable for all donor pancreata. The manual method is less expensive and more adaptable and enables islets to be isolated for research from most donor pancreata.

Key Words: human islets of Langerhans • human islet isolation • islet cell transplantation

Diabetes and Vascular Disease Research, Vol. 4, No. 1, 7-12 (2007)
DOI: 10.3132/dvdr.2007.010
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