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Endothelial nitric oxide synthase (eNOS) gene polymorphisms and their association with type 2 diabetes-related traits in Mexican Americans

Division of Nephrology, Department of Medicine, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78229, US.

Sobha Puppala

Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, US.

Nedal H Arar

Division of Nephrology, Department of Medicine, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78229, US.

Michael P Stern

Division of Clinical Epidemiology, Department of Medicine, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78229, US.

John Blangero

Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, US.

Ravindranath Duggirala

Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, US.

Hanna E Abboud

Division of Nephrology, Department of Medicine, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78229, US.

Genetic variants of the endothelial nitric oxide synthase (eNOS) gene such as T-786C, Glu298Asp and 27bp-VNTR have been examined for their association with type 2 diabetes (T2DM)-related traits in various populations but not in Mexican Americans. However, the results from such studies have been controversial. This study investigated whether these three polymorphisms are associated with T2DM and its related traits in Mexican Americans, a population at high risk for T2DM and its complications. The study participants (n=670; 39 families) were genotyped for the three polymorphisms using polymerase chain reaction followed by restriction fragment length polymorphism assay. Association analyses between these polymorphisms and T2DM and its related phenotypes were carried out using a measured genotype approach as implemented in the computer program SOLAR.

Of the variants examined, only the 27bp-VNTR variant exhibited significant association with high-density lipoprotein cholesterol (HDL-C) (p=0.04) and diastolic blood pressure (DBP) levels (p=0.02) after accounting for trait-specific covariates. The carriers of the rare allele (27bp-VNTR-4a) were found to have decreased HDL-C and increased DBP levels. In conclusion, of the genetic polymorphisms examined at the eNOS locus, only 27bp-VNTR appears to be a minor contributor to the variation in T2DM-related traits in Mexican Americans.

Key Words: association analyses • eNOS gene • genetic polymorphisms • Mexican Americans • type 2 diabetes

Diabetes and Vascular Disease Research, Vol. 5, No. 2, 109-113 (2008)
DOI: 10.3132/dvdr.2008.018


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